A glutamyl-tRNA reductase (EC 1.2.1.70) is an enzyme that catalyzes the chemical reaction

L-glutamate 1-semialdehyde NADP tRNAGlu ⇌ {\displaystyle \rightleftharpoons } L-glutamyl-tRNAGlu NADPH H

The 3 substrates of this enzyme are L-glutamate 1-semialdehyde, NADP , and tRNA(Glu), whereas its 3 products are L-glutamyl-tRNA(Glu), NADPH, and H .

This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the aldehyde or oxo group of donor with NAD or NADP as acceptor. The systematic name of this enzyme class is L-glutamate-semialdehyde: NADP oxidoreductase (L-glutamyl-tRNAGlu-forming). This enzyme participates in porphyrin and chlorophyll metabolism.

References

  • Von Wettstein D, Gough S, Kannangara CG (1995). "Chlorophyll Biosynthesis". Plant Cell. 7 (7): 1039–1057. doi:10.1105/tpc.7.7.1039. PMC 160907. PMID 12242396.
  • Pontoppidan B, Kannangara CG (1994). "Purification and partial characterisation of barley glutamyl-tRNA(Glu) reductase, the enzyme that directs glutamate to chlorophyll biosynthesis". Eur. J. Biochem. 225 (2): 529–37. doi:10.1111/j.1432-1033.1994.00529.x. PMID 7957167.
  • Heinz DW, Inokuchi H, Soll D, Jahn D (2002). "Escherichia coli glutamyl-tRNA reductase. Trapping the thioester intermediate". J. Biol. Chem. 277 (50): 48657–63. doi:10.1074/jbc.M206924200. PMID 12370189.



GlutamyltRNA (PDB ID 1G59). Left 2D linear model showing the

Figure 1 from Crystal structure of Arabidopsis glutamyltRNA reductase

Mechanism of glutamyltRNA synthetase (a) The glutamyltRNA synthetase

(PDF) Structure and function of glutamyltRNA reductase involved in 5

(PDF) Escherichia coli glutamyltRNA reductase Trapping the thioester